Àá½Ã¸¸ ±â´Ù·Á ÁÖ¼¼¿ä. ·ÎµùÁßÀÔ´Ï´Ù.
KMID : 0352720230470030458
Journal of Ginseng Research
2023 Volume.47 No. 3 p.458 ~ p.468
Ginsenoside Rg1 treatment protects against cognitive dysfunction via inhibiting PLC?CN?NFAT1 signaling in T2DM mice
Xianan Dong

Liangliang Kong
Lei Huang
Dong Ynmao
Xuewang Li
Liu Yang
Pengmin Ji
Weiping Li
Weizu Li
Abstract
Background : As a complication of Type II Diabetes Mellitus (T2DM), the etiology, pathogenesis, and treatment of cognitive dysfunction are still undefined. Recent studies demonstrated that Ginsenoside Rg1 (Rg1) has promising neuroprotective properties, but the effect and mechanism in diabetes-associated cognitive dysfunction (DACD) deserve further investigation.

Methods : After establishing the T2DM model with a high-fat diet and STZ intraperitoneal injection, Rg1 was given for 8 weeks. The behavior alterations and neuronal lesions were judged using the open field test (OFT) and Morris water maze (MWM), as well as HE and Nissl staining. The protein or mRNA changes of NOX2, p-PLC, TRPC6, CN, NFAT1, APP, BACE1, NCSTN, and A¥â1-42 were investigated by immunoblot, immunofluorescence or qPCR. Commercial kits were used to evaluate the levels of IP3, DAG, and calcium ion (Ca2+) in brain tissues.

Results : Rg1 therapy improved memory impairment and neuronal injury, decreased ROS, IP3, and DAG levels to revert Ca2+ overload, downregulated the expressions of p-PLC, TRPC6, CN, and NFAT1 nuclear translocation, and alleviated A¥â deposition in T2DM mice. In addition, Rg1 therapy elevated the expression of PSD95 and SYN in T2DM mice, which in turn improved synaptic dysfunction.

Conclusions : Rg1 therapy may improve neuronal injury and DACD via mediating PLC?CN?NFAT1 signal pathway to reduce A¥â generation in T2DM mice.
KEYWORD
Type II Diabetes Mellitus, Cognitive dysfunction, Ginsenoside Rg1, Calcium homeostasis, Phospholipase C
FullTexts / Linksout information
Listed journal information